Abstract
Ammonium sulphate fractionated pointed gourd (Trichosanthes dioica) peroxidase- concanavalin A (PGP-Con A) complex was entrapped into calcium alginate-pectin gel. Catalytic performance of immobilized PGP-Con A complex in dye decolorization was examined on repeated use and reusing after a prolonged period of storage. Immobilized and entrapped peroxidase preparation retained 59.6% of the original activity after a period of 50 d. Entrapped PGPCon A complex decolorized 91.2% and 82.1% of the initial color from DR19 and dye mixture [DR19+DB9] after 20 d, respectively. Considerable color removal was found even after 120 d and 80 d respectively, of operation of two reactor system and total organic carbon analysis was quite comparable to color loss. This study shows the efficacy, durability and sustainability of using immobilized T. dioica peroxidase in batch and continuous two reactor catalytic system for the removal of synthetic dyes from industrial effluents.
Highlights
Enzymes are environmental friendly but are capable of reducing hazardous wastes and key to new processes
The current study demonstrates a simple, inexpensive and high yield procedure for immobilization of glycosylated Trichosanthes dioica peroxidase
With 0.2 mL of Concanavalin A (Con A) the PGP-Con A complex expressed an activity of 79% which on entrapment into calcium alginate-pectin gel resulted in further decrease of peroxidase activity (Table 1)
Summary
Enzymes are environmental friendly but are capable of reducing hazardous wastes and key to new processes. Peroxidases are hemecontaining monomeric glycoproteins that oxidize a wide variety of molecules [1,2] These enzymes find wide range of applications in detoxification, dye decolorization and removal of various toxic organic pollutants which contaminate water and industrial effluents [3,4,5]. Several factors like shorter treatment period; operation of high and low concentrations of substrates; reduced lag phase of biomass, reduction in sludge volume and ease of controlling the process makes enzymatic treatment potentially useful as compared to microbial treatment These enzymes catalyze a variety of aromatic compounds in the presence of hydrogen peroxide, several limitations prevent the use of liquid form of enzymes as their stability and catalytic ability decreases with the complex nature of the effluents [9]. A comparative study on the reusability and storage stability of soluble and immobilized form of PGP (PGP-Con A complex and PGP-Con A-calcium alginate-pectin complex) has been presented for using such enzymes in effectively removing/minimising the color in industrial effluent contaminated with dyes
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