Abstract
Six isonitrogenous and isocaloric purified diets with different threonine concentrations (5, 10, 15, 20, 25, and 30 g kg−1) were fed to triplicate groups of Channa punctatus (6.24 ± 0.07 g) for 12 weeks to estimate dietary threonine requirement. Absolute weight gain, protein and threonine retention efficiency, feed conversion ratio and RNA/DNA ratio of trial diets with incremental threonine concentrations improved up to 20 g kg−1 dry diet. Carcass protein and moisture contents increased up to 20 g kg−1 dietary threonine. However, carcass fat also showed a reverse pattern, increasing threonine concentrations up to a 20 g kg−1 dry diet. Hemoglobin (Hb), red blood corpuscles (RBCs) and hematocrit (Hct) value improved significantly (p < .05) up to 20 g kg−1. Total serum protein, superoxide dismutase (SOD), catalase (CAT), and lysozyme was found to increase significantly up to 20 g kg−1 dry diet, and a significant fall was recorded at a higher threonine level (Th30). Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) concentrations declined significantly with dietary threonine levels up to 20 g kg−1. Quadratic regression analysis of absolute weight gain, feed conversion ratio, protein, and threonine retention efficiency data against incremental threonine levels projected the optimum requirement between 22.2 and 23.0 g kg−1 dry diets.
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