Dietary therapeutic treatment of renal carcinoma cell lines by down-regulating cFlip, Mcl-1, Bcl-XL and STAT3 gene expression under the influence of up-regulated Bax and intrinsic apoptotic pathway

Abstract

Renal cell carcinoma (RCC) is a lethal genitourinary cancer and accounts 3% of total cancers worldwide. Various pleiotropic cytokines, i.e activator of signal transducer and activator of transcription 3 (STAT3), Cellular FLICE (FADD-like IL-1β-converting enzyme)-inhibitory protein (c-FLIP) and interleukin-6 (IL-6) could act to block the process of apoptosis in renal cancinoma cells, trigering its growth and proliferation. Their over-expression could activate several anti-apoptotic factors, hence it is important to block or down-regulate such factors in order to activate apoptosis in cancer cells. Up till now, several chemo and radiotherapies are being considered to combat renal carcinoma cells; however, there is a strong need to get some dietary therapeutic treatment. Hence, this study has used gastrointestinal digests of preserved eggs (GID-PE) for the apoptosis of Caki-1, ACHN and 786-O cells lines, in order to get insights about apoptosis in their mitochondrial pathway before and after treatment with (GID-PE). Moreover, changes in morphology accompanied by apoptosis analysis with different treatments have been carried out. Furthermore, ELISA analysis and mRNA expression of certain pro-inflammatory and anti-inflammatory cytokines were determined before and after treatment with GID-PE. Furthermore, the specific anti-apoptotic genes, i.e cFlip, Mcl-1, Bcl-XL and STAT3 were examined for their down-regulated expression after different treatments with GID-PE. Moreover, Bax expression acting as a pro-apoptotic gene was up-regulated up to some extent after treatments with GID-PE. Hence, GID-PE was confirmed to give anti-inflammatory effects in renal carcinoma cells could be used as health beneficial food product with anti-cancer properties.