Dietary supplementation with glutamine and γ-aminobutyric acid improves growth performance and serum parameters in 22- to 35-day-old broilers exposed to hot environment.

Abstract

This study was designed using 360 21-day-old chicks to determine the influences of diet supplementation with glutamine (5g/kg), γ-aminobutyric acid (GABA, 100mg/kg) or their combinations on performance and serum parameters exposed to cycling high temperatures. From 22 to 35days, the experimental groups (2 × 2) were subjected to circular heat stress by exposing them to 30-34°C cycling, while the positive control group was exposed to 23°C constant. The blood of broilers was collected to detect serum parameters on days 28 and 35. Compared with the positive control group, the cycling high temperature decreased (p<0.05) the feed consumption, weight gain and serum total protein (TP), glucose, thyroxine (T4), insulin, alkaline phosphatase (ALP), glutamine, GABA and glutamate levels, while increased (p<0.05) the serum triglyceride (TG), corticosterone (CS), glucagon (GN), creatine kinase (CK), glutamic oxaloacetic transaminase (GOT), nitric oxide synthase (NOS), glutamate pyruvate transaminase (GPT) and lactate dehydrogenase (LDH) levels during 22-35days. However, dietary glutamine (5g/kg) increased (p<0.05) the feed consumption, weight gain and serum levels of glutamine, TP, insulin and ALP, but decreased (p<0.05) the serum TG, CK, GOT, NOS and GPT levels. Diet supplemented with GABA also increased (p<0.05) weight gain and the serum levels of TP, T4, ALP, GABA and glutamine. In addition, the significant interactions (p<0.05) between glutamine and GABA were found in the feed consumption, weight gain and the serum ALP, CK, LDH, GABA, T3 and T4 levels of heat-stressed chickens. This research indicated that dietary glutamine and GABA improved the antistress ability in performance and serum parameters of broilers under hot environment.