Dietary Supplementation of Vine Tea Ameliorates Glucose and Lipid Metabolic Disorder via Akt Signaling Pathway in Diabetic Rats.

Jiamei Xiang,Baoping Jiang,Qiuyue Lv,Liang Le,Lijia Xu,Fan Yi,Yanjun Song,Peigen Xiao

doi:10.3390/molecules24101866

Abstract

A traditional Chinese tea with many pharmacological effects, vine tea (VT) is considered a potential dietary supplement to improve type 2 diabetes (T2D). To investigate the effect and mechanism of VT on glucose and lipid metabolic disorders in T2D rats, Wistar rats fed a normal diet served as the normal control, while rats fed a high-fat diet combined with low-dose streptozotocin (STZ)-induced T2D were divided into three groups: The model group (MOD); the positive control group (MET, metformin at 200 mg/kg/d); and the VT-treated group (VT500, allowed to freely drink 500 mg/L VT). After four weeks of intervention, biochemical metrics indicated that VT significantly ameliorated hyperglycemia, hyperlipidemia and hyperinsulinemia in T2D rats. Metabolomics research indicated that VT regulated the levels of metabolites closely related to glucose and lipid metabolism and promoted glycogen synthesis. Furthermore, VT had a significant influence on the expression of key genes involved in the Akt signaling pathway, inhibited gluconeogenesis through the Akt/Foxo1/Pck2 signaling pathway, and reduced fatty acid synthesis via the SREBP1c/Fasn signaling pathways. In conclusion, VT has great potential as a dietary supplement to ameliorate glucose and lipid metabolic disorders via the Akt signaling pathway in T2D rats.

Highlights

In the past three decades, the incidence of diabetes mellitus has dramatically increased
The present results indicated that the VT500 group had lower serum levels of TG, CHO, low-density lipoprotein (LDL), and nonesterified fatty acids (NEFA) and higher levels of HDL than the model group (MOD) group, suggesting that vine tea (VT) has a good effect on improving dyslipidemia
Our results indicated that VT significantly reduced the levels of fasting blood glucose (FBG), hemoglobin A1c (HbA1c) and insulin, improved glucose tolerance, and accelerated insulin sensitivity (Figure 2)

Summary

Introduction

In the past three decades, the incidence of diabetes mellitus has dramatically increased. There are approximately 415 million people with diabetes mellitus, which is considered a major cause of death [1,2]. Patients diagnosed with type 2 diabetes (T2D) account for more than 90% of all diabetic patients [1]. Asia is a major contributor to the rapid emergence of T2D, with China and. India as representatives [2]. T2D is generally considered to be a chronic metabolic disorder. Improving the metabolic dysfunction of glucose and lipid is an important direction for T2D prevention and treatment [3,4,5,6,7]

Methods

Results

Discussion

Conclusion