Dietary Acid and Alkali Loading Do Not Alter Taurine Uptake by Renal Proximal Tubule Brush Border Membrane Vesicles in Kittens

Abstract

Kittens adapted to a purified control diet containing 43.5% soy protein plus 0.15% taurine were randomly divided into three groups: control, acid-loaded or alkali-loaded. For dietary acid or alkali loadings, 2% NH4Cl or 0.87% NaHCO3 plus 0.87% KHCO3 were added to the control diet. Acid-loaded, control and alkali-loaded kittens had venous blood pH of 7.33 +/- 0.01, 7.37 +/- 0.02 and 7.39 +/- 0.02, and urine pH of 5.5 +/- 0.1, 7.2 +/- 0.1 and 8.1 +/- 0.1, respectively. After 6 wk of the dietary treatment, the plasma taurine concentrations of acid-loaded, control and alkali-loaded kittens were 74 +/- 4, 77 +/- 7 and 87 +/- 8 mumol/L (P greater than 0.05) respectively. Compared with the control group, dietary acid or alkali loading did not significantly change the taurine uptake by brush border membrane vesicles (BBMV) when BBMV were at the same in vitro pH (7.35). However, when the pH of the medium was changed to 8.0 for the alkali-loaded group or to 5.5 for the acid-loaded group, the initial taurine uptake was significantly elevated (55%) or decreased (31%) (P less than 0.05), respectively, compared with the values for the control group at pH 7.35. When BBMV prepared from the same group were tested at different pH levels (8.0 vs. 7.35 vs. 5.5), the initial uptake significantly increased (30%) at pH 8.0 and decreased (37%) at pH 5.5 compared with that observed at pH 7.35 (P less than 0.05), regardless of the acidity of the diet.(ABSTRACT TRUNCATED AT 250 WORDS)