Diagnostic value of hTERCgene expression in cervical intraepithelial neoplasia in gynecologic cytology

Abstract

：Objective To investigatethe expression of human telomerase RNA component (hTERC)gene in cervical scraping samples,and the relationship between hTERC gene and cervical lesions. Methods The fluorescent insitu hybridization (FISH) was used to detect the expression of hTERC oncogene in cervicalscraping samples in 100 cases, including 76 cases with cervical intraepithelial neoplasia,meanwhile, high risk human papillomavirus (HR-HPV) were detected by Hybrid Capture 2 (HC2). The other 24 cases were negative for intraepithelial lesion (NILM). Results The positiveexpression rates of the hTERC gene in NILM,ASCUS, ASC-H, LSIL, HSIL and cervical cancerwere 0, 32.25 %, 75.00 %, 35.71 %, 81.81 % and 100.00 % respectively. hTERC gene positiveexpression in normal group was less significantly than that in abnormal groups (P<0.05). There was a significantly difference between HSIL and ASCUS, LSIL (x2 = 6.1736,x2 =5.0004, P <0.05). The positive expression rates of the HR-HPV in ASCUS, ASC-H, LSILand HSIL or higher lesions were 54.80 %, 75.00 %, 60.70 %, 90.90 % and 100.00 %respectively. HR-HPV positive expression in HSIL or higher lesions were more significantlythan that in ASCUS group (x2=4.1767, P<0.05). Conclusion The overexpression of hTERCgene may play an important role in cervical carcinogenesis, therefore it can be used as abetter tumor marker for prospective in early cervical cancer progresses.