Assessment on application value of combination of TCT, HPV, c-MYC and hTERC genes to screen cervical cancer

Abstract

Objective To investigate the application value of combination of thinprep cytologic test (TCT), human papilloma virus (HPV), c-MYC and human chromosome telomerase gene (hTERC) genes to screen cervical cancer. Methods A total of 230 cases of the study objects was detected with TCT and HP, respectively. Amplification of c-MYC and hTERC genes was tested with fluorescence in situ hybridization (FISH) method. The histopathological results were the gold standard, and the sensitivity, specificity and accuracy of cervical intraepithelial neoplasia (CIN) Ⅱ/Ⅲ and squamous cell carcinoma (SCC) were compared with the four combined detection. Results Of 230 screened patients, there were 124 cases of TCT positive, 155 cases of HPV positive, c-MYC gene amplified in 118 cases, and hTERC gene amplified in 128 cases. When TCT, HPV, c-MYC, and hTERC were used alone, the highest sensitivity was HPV (84.5%), the highest specificity was c-MYC (97.6%), and the highest accuracy was hTERC (85.2%). When the three indexes were used in coordination with each other, the sensitivity and accuracy of TCT+ HPV+ hTERC were the highest (98.6% and 90.9%), and the specificity of TCT+ c-MYC+ hTERC was the highest (79.3%). When the four indexes were used together, the sensitivity was 98.6%, the specificity was 72.0%, and the accuracy was 89.1%. Conclusions Combined examination can improve the sensitivity and accuracy of screening cervical lesions, and the three combination of TCT+ HPV+ hTERC had the best effect, and c-MYC gene detection had the highest specificity. Key words: Biopsy; Papillomaviridae; Genes, myc; Telomerase; Uterine cervical neoplasms/DI