Diagnostic value of 4kinds of serum autoantibodies in nasopharyngeal carcinoma

Abstract

：Objective To analyze the diagnostic value of 4 serumautoantibodies in nasopharyngeal carcinoma patients (anti-Rho-GDI-2, -HSP70, -CK19 and-LAP3 antibodies) using a serological proteomic approach. Methods The conditions forblocking, antigen concentrations and serum dilution were optimized to establish the ELISAmethod for detection of serum autoantibodies for Rho-GDI-2, HSP70, CK19 and LAP3. Theserum concentrations of these autoantibodies were detected by the established method in 36NPC patients, 20 other cancer patients and 20 healthy individuals. The statisticalanalysis was used to evaluate the diagnostic value of the 4 serum autoantibodies. ResultsThe concentrations of 4 serum autoantibodies in the NPC patients were significantly higherthan those in the healthy individuals(A450 values in NPC group of CK19, Rho-GDI-2, HSP70,LAP3 were 0. 188 8 ±0.047 3, 0.240 5 ± 0.024 7, 0. 116 8 ±0.025 3, 0.276 9 ±0.044 2repectively. A450 values in healthy control group were 0.010 5 ±0.004 4, 0. 105 3 ±0.016 9, 0. 098 6 ± 0. 014 0, 0. 149 8 ± 0. 033 1 respectively. F values were 4. 869, 15.919, 10. 331,6. 369, respectively. P values were 0. 010, 0. 000, 0. 000, 0. 003,respectively). The sensitivities of antiRho-GDI-2, anti-HSP70, anti -CK19 and anti -LAP3autoantibodies for diagnosis of NPC was 85.0%,75.0%, 75.0% and 75.0%, respectively, andthe specificities were 58.9%, 80. 4%, 58. 9% and 57. 1%, respectively, anti-Rho-GDI-2 andanti-HSP70 achived the highest sensitivity and specificity respectively when single markerwas analyzed. The sensitivity and specificity were 94. 4% and 95. 0%respectively when 4markers was combined for analysis. Conclusion Detection of serumanti-Rbo-GDI-2,anti-HSP70, anti-CK19 and anti-IAP3 auotantibedios by ELISA may be of helpin the screening and diagnosis for NPC. Key words: Nasopharyngeal carcinoma; ELISA; Autoantib