Diagnostic study to detect toxoplasmosis in some Iraqi sheep

Abstract

Parasites are cause a lot of important and serious diseases which widespread to peoples and animals all over the world, vector for disease to humans by consumption of meat sheep fresh as liver or not cooked well meat, so in this way the toxoplasmosis in sheep considered one of the causes economic and social damage and important sources of transfer this infection human, for this fact we must value economic and social damage by investigate the incidence of this disease in sheep, and using accurate methods for detection of this infection in blood fluids or tissue of sheep, so in this study we using immunological and molecular methods to detection of the parasite in sheep blood specimens in Al-Shamiya city/Al-Qadisiyah government/Iraq, the results explained presence that antibodies in 14 samples (14%) below level significant (P≤ 0.05) by using Rapid Test Cassette, while the results depending on (RT PCR) for detection of specific gene of parasite B1 gene in blood specimens showed to (9 of 100)(9%) positive infection results in examined sheep. from these results, we can give simple picture ensured rule the infected sheep in transmission the disease to human although a low percentage of positive infection, presence the disease in sheep indicator to role sheep to transfer the disease to the human an achieved the economic and social damage.