Abstract
Part I. Introduction. The Antibody Molecule, Andrew J. T. George. Polyclonal and Monoclonal Antibodies, Mary A. Ritter. Engineering Antibody Molecules, Rakesh Verma and Ekaterini Boleti. Phage Display Technology, Michael Johns. Part II. Antibodies in Medicine. Prospects for the Application of Antibodies in Medicine, Herman Waldmann. Antibodies for Neoplastic Disease: Solid Tumors, Ian T. W. Matthews. The Application of Monoclonal Antibodies in the Treatment of Lymphoma, Martin J. Glennie, Jamie Honeychurch, Ruth R. French, and Alison L. Tutt. Antibodies for Inflammatory Disease: Effector Cells, Richard Smith. Antibodies for Inflammatory Disease: Cytokines, Peter C. Taylor. Antibodies for Transplantation, Denise L. Faustman. Antibody-Based Therapies in Infectious Diseases, H. Barbaros Oral and Cezmi A. Akdis. Antibodies in Nuclear Medicine, A. Michael Peters. Animal Models for Tumor Localization, Gail Rowlinson-Busza. Antibodies for Immunoassays, David J. Newman. Part III. Ethics and Industry. Intellectual Property, William M. Brown. From Laboratory to Clinic: The Story of CAMPATH-1, Geoff Hale and Herman Waldmann. Part IV. Production and Purification. Production of Monoclonal Antibodies, Jona Freysdottir. Purification of Monoclonal Antibodies Using Protein A/G, Bridget Heelan. Preparation of Monoclonal Antibodies Using Ion Exchange Chromatography, Maureen Power. Quality Control of Raw Materials, Patrick Harrison and Geoff Hale. Cell Banks and Stability of Antibody Production, Pru Bird and Geoff Hale. Measurement of Endotoxin, Jenny Phillips, Patrick Harrison, and Geoff Hale. Aseptic Vial Filling, Kuldip Bhamra, Patrick Harrison, and Geoff Hale. Measurement of Antibody Concentrations by Hemagglutination, Jenny Phillips and Geoff Hale. Part V. Modification of Antibodies. Enzymatic Digestion of Monoclonal Antibodies, Sarah M. Andrew. How to Make Bispecific Antibodies, Ruth R. French. Radiolabeling Monoclonal Antibodies, Calvin S. R. Gooden. Determination of theImmunoreactivity of Radiolabeled Monoclonal Antibodies, Gail Rowlinson-Busza. Use of Biosensors to Measure the Kinetics of Antibody-Antigen Interactions, Andrew J. T. George. Part VI. Application of Antibodies In Vitro. How to Set Up an ELISA, Bill Jordan. Measurement of HAMA and Anti-Idiotypic Antibodies, Steve Nicholson. SDS-PAGE and Western Blotting, Abdulhamid A. Al-Tubuly. Flow Cytometric Analysis, Paul F. McKay. Immunocytochemistry, Susan Van Noorden. Immunolabeling for Electron Microscopy, Catherine E. Sarraf. Part VII. Antibody Engineering. PCR of the V-Region, Rakesh Verma. Phage Display Technology: Protocols, Michael Johns and Donald B. Palmer. Index.
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