Diagnostic and prognostic value of serum miR-9-5p and miR-128-3p levels in early-stage acute ischemic stroke

Qi Wang,Fei Wang,Fengwei Fu,Jinlin Liu,Weilu Sun,Yongqing Chen

doi:10.6061/clinics/2021/e2958

Abstract

OBJECTIVES:To investigate the clinical utility of serum microRNA levels (miR-9-5p and miR-128-3p) in the diagnosis and prognosis of early-stage acute ischemic stroke (AIS).METHODS:We compared the differences in serum miR-9-5p and miR-128-3p levels between patients with AIS and healthy individuals (controls). The serum levels of miR-9-5p and miR-128-3p were quantified using quantitative real-time PCR, and the association of each miRNA with AIS was determined using receiver operator characteristic curve analysis. The predictive value of these indices in the diagnosis of early-stage AIS was evaluated in conjunction with that of computed tomography findings and neuron-specific enolase levels. The prognosis of patients with AIS was evaluated three months after their discharge from hospital using the modified Rankin scale, which classifies the prognosis as either favorable or poor. Logistic regression analysis was used to analyze the correlation between miR-9-5p and miR-128-3p levels and patient prognosis.RESULTS:The serum levels of miR-9-5p and miR-128-3p were upregulated in patients with AIS relative to those in healthy individuals. A pronounced correlation was identified between serum miR-9-5p and miR-128-3p levels and patient prognosis, with high levels of both miRNAs being associated with poor patient outcomes.CONCLUSION:Assessment of serum miR-9-5p and miR-128-3p levels is important for the early diagnosis and prognosis of AIS.

Highlights

Acute ischemic stroke (AIS) accounts for 70% of all stroke cases and is associated with markedly high patient disability and mortality rates [1]
Baseline data The patient and control groups were compared in terms of sex ratio, age, body mass index (BMI), laboratory indicators, and incidence of complications
The levels of miR-9-5p and miR-128-3p were found to be correlated with blood pressure, BMI, low-density lipoprotein (LDL) levels, hypertension, and hyperlipidemia

Summary

Introduction

Acute ischemic stroke (AIS) accounts for 70% of all stroke cases and is associated with markedly high patient disability and mortality rates [1]. Diagnosis and timely intervention would significantly reduce these rates and improve the prognostic outcomes and quality of life of patients with AIS [2,3]. AIS is diagnosed using computed tomography (CT). In the early stages of the disease, CT cannot identify any abnormalities in approximately 40–50% of the patients with AIS [4]. Interleukin-6 [5,6], neuron-specific enolase (NSE) [7], glial fibrillary acidic protein [8], and 25-hydroxyvitamin D [9] are associated with.

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