Abstract
Methyglyoxal (MGO) is an important pathological factor for diabetic cardiovascular complications. Conventional methods for MGO detection in biological samples, such as high performance liquid chromatography (HPLC)-UV spectrometry, LC-fluorescence spectrometry, and HPLC-mass spectrometry, are time-consuming, high-cost, and complicated. Here, we present a method for MGO quantitative detection based on far-IR spectral analyses. Our method uses o-phenylenediamine (OPD) to produce a chemical reaction with MGO, which results in multiple fingerprint feature changes associated with the molar ratio of MGO and OPD. We use the linear relationship between MGO concentration and peak intensity of the reaction product to quantitatively determine MGO concentration. The corresponding linear detectable range is 5∼2500 nmol/mL nmol per mL with a correlation coefficient of 0.999. This quantitative method is also tested by blood samples with adjusted MGO concentrations, and shows 95% accuracy with only 30s testing time. Our method provides a fast, simple and economical approach to determining MGO concentration in blood.
Highlights
Diabetes mellitus is estimated to affect more than 415 million adults worldwide
The fingerprint spectrum of pure MGO was measured in the range from 30 to 500 cm−1 [ Fig. 1(a)]
The chemical reaction between MGO and o-phenylenediamine (OPD) is used to induce multiple fingerprint feature changes according to the molar ratio change between MGO and OPD
Summary
Diabetes mellitus is estimated to affect more than 415 million adults worldwide. The prevalence is increasing, and it is expected that more than 640 million adults will have diabetes by 2040 [1]. Patients with diabetes are at high risk for adverse outcomes from atherosclerotic cardiovascular disease [2,3], heart failure [4], and renal disease [5]. The main diagnostic methods for diabetic cardiovascular complications are coronary angiography, magnetic resonance (MR) imaging, and computed tomographic (CT) angiography. Coronary angiography is the golden standard for diagnosing diabetic cardiovascular complications, but it is invasive and its intravascular 2D imaging cannot provide enough information for accurate surgical design [6]. While for MR imaging and CT angiography, gadolinium-based contrast agents are unavoidable and the cost is high [7,8]
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