Abstract
The use of quantitative real time polymerase chain reaction (qPCR) for herpesvirus detection has improved the sensitivity and specificity of diagnosis, as it is able to detect shedding episodes in the absence of clinical lesions and diagnose clinical specimens that have low viral loads. With an aim to improve the detection and quantification of herpesvirus by qPCR, synthetic standard curves for human herpesvirus 1 and 2 (HHV-1 and HHV-2) targeting regions gD and gG, respectively, were designed and evaluated. The results show that synthetic curves can replace DNA standard curves in diagnostic herpes qPCR.
Highlights
The human herpesvirus or herpes simplex virus (HHV or HSV) is a neurotropic virus that has two distinct serotypes, human herpesvirus 1 and 2 (HHV-1 and human herpesvirus-2 (HHV-2))
Human herpesvirus-1 (HHV-1) was considered the main cause of orolabial lesions, and HHV-2 was most commonly associated with genital infections
HHV-1 is increasingly being detected in genital lesions, and HHV-2 in orolabial lesions (Bhattarakosol et al 2005)
Summary
The human herpesvirus or herpes simplex virus (HHV or HSV) is a neurotropic virus that has two distinct serotypes, human herpesvirus 1 and 2 (HHV-1 and HHV-2). In qPCR, the viral load is measured as the copy number per cell or percentage of total DNA by using a standard curve. A standard curve is generated by qPCR using a dilution series of a DNA template, which is commonly generated from plasmid DNA or DNA oligonucleotides (Tourinho et al 2015).
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