Diagnosis and analysis of allergen with skin prick test and serum specific IgE detection in allergic disease

Abstract

Objective To analyse the major allergen with skin prick test(SPT) in Xi'an area in different allergic diseases and evaluate of the results of SPT with specific IgE detection with UniCAP 100 in vitro. Methods 679 patients with allergic disease from our allergic out-patient department were accepted SPT with 19 kinds of common allergens. And the positive rates in different allergic diseases were compared. 116 cases of these allergic diseases patients were measured serum specific IgE against dematophagoids farinae and the results were comparedwith SPT results. Results ①Dematophagoids farinae and dematophagoids pteronyssinus were the major allergen inall kinds of allergic diseases with the SPT positive rate being 49.78% and 43. 00%. Artemisia pollen, molds, Platanus acerifolia pollen and Tree Ⅰ pollen were also the major allergen in turns with the SPT positive rate than 10%. These allergens were all the major allergens in Xi'an area in allergic diseases. ②The SPT positive rates of feather, egg, milk, Artemisia pollen, shrimp and peanut were different in different kinds of allergic diseases significantly while the others had no significant difference in different kinds of allergic diseases. Inhalant allergens were the major allergens in athmatie and allergic rhinitis patients. Whereas in urticaria and allergic purpura patients some edibility allergens such as egg, milk, shrimp and peanut were also the major allergens aparting from theinhalant allergens. ③The SPT diagnostic value with + + or more as diagnostic criteria was higher than that ofwith + or more as diagnostic criteria when the serum specific IgE was used as golden criteria. The specific IgEquantitation and the diameter of SPT wheals were logarithmic correlated with each other (r=0. 629, P<0. 05). Conclusions Dematophagoids farirnae, dematophagoids pteronyssinus, Artemisia pollen, molds, Platanus acerifolia pollen and Tree Ⅰ pollen were the major allergens of allergic diseases in Xi'an area. The major allergen was slightly different in different allergic diseases. The specific IgE quantitation and the diameter of SPT wheals were logarithmic correlated with each other and the two kinds of tests could complement with each other. Key words: Allergic disease; Allergen; Skin prick test; Specific IgE