Abstract
In this study, a new STRtyper-27 system, including the same Yfiler Plus loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, Y-GATA H4, DYS449, DYS460, DYS481, DYS518, DYS533, DYS570, DYS576, DYS627 and DYF387S1a/b), was established using a set of 5 fluorescent dye labels. Primers, internal size standard, allelic ladders and matrix standard set were designed and created in-house for this multiplex system. This paper describes the validation studies conducted with the STRtyper-27Y system using a 3130XL genetic analyzer for fragment length detection that included the analysis of the following parameters and aspects: sensitivity, species specificity, inhibition, haplotype concordance, precision, stutter, DNA mixtures, and stability studies with crime scene samples. The studies demonstrated, that the STRtyper-27Y system provided equivalent overall performance comparable to the latest Yfiler Plus kit, but with enhanced compatibility in terms of instrument platforms and software allowing forensic laboratories to conduct its forensic application and evaluate its performance, all in their own 5 dye Y-STR chemistry system /environment without software or instrument upgrades.
Highlights
Locus DYS456* DYS576*,★ DYS570★ DYS481 DYF387S1★ DYS627★ DYS458* DYS460* DYS437 DYS439 DYS392 DYS385a/b DYS393* DYS391* DYS390 DYS635 DYS449★ DYS533 DYS438* DYS389I* DYS448 DYS389II DYS19 Y-GATA-H4 DYS518★
A new STRtyper-27 multiplex system that allows co-amplification of the same 27 Y-specific short tandem repeat (Y-STR) loci included in the Yfiler Plus kit was developed and detected by existing instrument platforms and software so that forensic laboratories can conduct experiments, evaluate its performance, and compare results to the latest commercial available Y-STR kit, all in their own 5-dye Y-STR chemical environment
Full and clean profiles were obtained when PCR was performed with annealing temperatures between 59 °C and 63 °C, and fragment dropouts were observed at the annealing temperature of 65 °C (Supplementary Fig. S1)
Summary
Locus DYS456* DYS576*,★ DYS570★ DYS481 DYF387S1★ DYS627★ DYS458* DYS460* DYS437 DYS439 DYS392 DYS385a/b DYS393* DYS391* DYS390 DYS635 DYS449★ DYS533 DYS438* DYS389I* DYS448 DYS389II DYS19 Y-GATA-H4 DYS518★. For details on the marker’s repeat structure, genomic location, final concentration, observed allelic ranges, haplotypes of control DNA 9948, 007 and dye labeling refer to the Table 1.
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call