Abstract
The biochemical basis of light-dependent chlorophyll (Chl) biosynthesis in angiosperms, the most highly evolved group of higher plants, is the strictly light- and NADPH-dependent enzymatic reduction of protochlorophyllide (Pchlide), a late Chi biosynthetic precursor (1). Pchlide is converted to chlorophyllide by NADPH:Pchlide oxidoreductases A and B (PORA and PORB; EC 1.3.1.33), two structurally related but differentially regulated enzymes that are encoded in the nucleus, translated in the cytosol, and ultimately imported into plastids (2–4).
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