Developmental neurotoxic effects of chronic endosulfan exposure in the zebrafish larvae

Abstract

Event Abstract Back to Event Developmental neurotoxic effects of chronic endosulfan exposure in the zebrafish larvae Nurul F. Ramlan1, NSA Mohd Sata1 and Wan Norhamidah Wan Ibrahim1* 1 Universiti Putra Malaysia, Department of Biology, Faculty of Science, Malaysia Endosulfan was listed as a member of Persistent Organic Pollutants (POP) and the usage was banned, yet this pesticide is still widely used in many developing countries. The usage of this pesticide can reach aquatic system through runoff from agricultural areas. Due to their persistent characteristic in the environment, human population are posed to continuous exposure to endosulfan. Consequently, endosulfan was found in the cord blood and breast milk of pregnant women, thus may induced adverse effects to the developing nervous system. In this study, we focused to determine the developmental neurotoxicity effects of chronic embryonic exposure to endosulfan in the zebrafish larvae. The zebrafish embryos were exposed to several concentrations of endosulfan in a semi static condition, where half of the medium was renewed for every 24 h from 5 hours post fertilization (hpf) until hatching. We measured spontaneous tail coiling at 24 hpf; heart rate at 48 hpf and mortality rate at 72 hpf. We found that, endosulfan decreased the spontaneous tail coiling, heart rate and increased the total percentage of mortality as the concentration increased. At 6 days post fertilization (dpf), we measured the swimming activity of individual larva and found that endosulfan significantly decreased the swimming activity behaviour at 40nM-200nM. The zebrafish larvae exposed to endosulfan higher than 200nM were unable to survive until 6 dpf. We further evaluated anxiety behaviour in the group of 5 larvae at 6 dpf exposed to 200nM endosulfan, with and without visual stimuli. Caffeine (100mg/L) was used as an anxiogenic drug. Caffeine increased the anxiety behaviour while endosulfan decreased the anxiety behaviour in the zebrafish larvae. Our result showed that zebrafish embryos can be an alternative research model to study the developmental neurotoxicity testing at high-throughput screenings. Keywords: Zebrafish, in vivo, pesticide, behaviour, Neurotoxic Conference: 14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016. Presentation Type: Poster Presentation Session Topic: 14th Meeting of the Asian-Pacific Society for Neurochemistry Citation: Ramlan NF, Mohd Sata N and Wan Ibrahim W (2016). Developmental neurotoxic effects of chronic endosulfan exposure in the zebrafish larvae. Conference Abstract: 14th Meeting of the Asian-Pacific Society for Neurochemistry. doi: 10.3389/conf.fncel.2016.36.00165 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Aug 2016; Published Online: 11 Aug 2016. * Correspondence: Dr. Wan Norhamidah Wan Ibrahim, Universiti Putra Malaysia, Department of Biology, Faculty of Science, UPM Serdang, Selangor, 43400, Malaysia, wnwi@upm.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Nurul F Ramlan NSA Mohd Sata Wan Norhamidah Wan Ibrahim Google Nurul F Ramlan NSA Mohd Sata Wan Norhamidah Wan Ibrahim Google Scholar Nurul F Ramlan NSA Mohd Sata Wan Norhamidah Wan Ibrahim PubMed Nurul F Ramlan NSA Mohd Sata Wan Norhamidah Wan Ibrahim Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.