Chronic embryonic exposure of arsenic trioxide induced alteration on motor functions and anxiety-like esponses in zebrafish (Danio rerio) larvae

Abstract

Event Abstract Back to Event Chronic embryonic exposure of arsenic trioxide induced alteration on motor functions and anxiety-like esponses in zebrafish (Danio rerio) larvae Noraini Abu Bakar1, Nurul Farhana Ramlan1 and Wan Norhamidah Wan Ibrahim1* 1 Universiti Putra Malaysia (UPM), Department of Biology, Faculty of Science, Malaysia Arsenic trioxide (As2O3) is a heavy metal that is widely used for therapeutic purpose and is found ubiquitously in the environment. Currently, information about the adverse effects associated with exposure to low levels of inorganic arsenic on the developing organisms is limited. We examined the effects of embryonic exposure of As2O3 on motor functions and anxiety-like behaviors in the developing zebrafish. The embryos were exposed to 4 different concentrations (20, 30, 40, 50 µM) starting from 5 hpf until hatching (72 hpf) in a semi-static condition. The control groups were maintained in the embryo media. The mortality rate increased in a dose dependent manner. We found that chronic embryonic exposure to 30 and 40 µM decreased the number of tail coiling, heartbeat, and swimming activity. Meanwhile, exposure 20 µM did not produce any significant alteration in these parameters. To further understand the effects ofAs2O3 interferences on the development of anxiety-related behavior, we chose 30 µM As2O3 exposed larvae since the 40 µM As2O3 achieved 100% mortality before the behavioral recording was conducted on 6 dpf. However, we found no alterations on thigmotaxis, avoidance response, speed and percentage of resting of 6 dpf larvae. Since we found no alterations in all parameters measured for anxiety related behavior, we evaluated the apoptosis in the eye of the larvae using acridine orange. We found a significant increase in the apoptotic signal particularly in the eye region. These results showed that exposure to microconcentration of As2O3 do not alter the anxiety related responses, yet, inducing apoptosis in the retina and lens, which may contribute to visual deficit. The potential underlying mechanisms and implication of As2O3 on visual acuity, motor performance and anxiety-like responses need further investigation. Keywords: Anxiety, Zebrafish, in vivo, heavy metal, behavioural assessment Conference: 14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016. Presentation Type: Poster Presentation Session Topic: 14th Meeting of the Asian-Pacific Society for Neurochemistry Citation: Abu Bakar N, Ramlan N and Wan Ibrahim W (2016). Chronic embryonic exposure of arsenic trioxide induced alteration on motor functions and anxiety-like esponses in zebrafish (Danio rerio) larvae. Conference Abstract: 14th Meeting of the Asian-Pacific Society for Neurochemistry. doi: 10.3389/conf.fncel.2016.36.00194 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Aug 2016; Published Online: 11 Aug 2016. * Correspondence: Dr. Wan Norhamidah Wan Ibrahim, Universiti Putra Malaysia (UPM), Department of Biology, Faculty of Science, Serdang, Selangor, Malaysia, wnwi@upm.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Noraini Abu Bakar Nurul Farhana Ramlan Wan Norhamidah Wan Ibrahim Google Noraini Abu Bakar Nurul Farhana Ramlan Wan Norhamidah Wan Ibrahim Google Scholar Noraini Abu Bakar Nurul Farhana Ramlan Wan Norhamidah Wan Ibrahim PubMed Noraini Abu Bakar Nurul Farhana Ramlan Wan Norhamidah Wan Ibrahim Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.