Abstract
An HPTLC technique for simultaneous estimation of Valsartan and Hydrochlorothiazide in a pharmaceutical combination dose form must be verified for stability indication. For the separation of VAL and HTZ using TLC plates coated with aluminium supported silica gel 60 F254, a high-performance thin layer chromatographic (HPTLC) technique has been devised. For the separation of two medicines, a variety of mobile phases were tried and tested. It was decided to use chloroform: ethyl acetate: ethanol/tetrahydrofuran as the final mobile phase for analysis. 247 nm was chosen as the detecting wavelength for both compounds since it exhibited highest absorption. As per the ICH recommendations, the devised technique was validated. Under varied stress settings, medicines were forced to degrade, and the stability of pharmaceuticals was assessed using the HPTLC technique. The separation of VAL and HTZ using HPTLC was a great success. A low percent RSD was determined by testing a variety of factors, and the stability experiments showed that the medicines and degraded products could be separated with an acceptable percent drug recovery profile. Rapid, accurate, and reproducible are some of the characteristics of the devised technique, which proves its appropriateness for valsartan and hydrochlorothiazide stability assessment.
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