Abstract
An accurate, simple reproducible and sensitive method for the determination of sumatriptan succinate was developed and validated. According to this, sumatriptan succinate was determined using a UV spectrophotometric (UVS) technique and the results were compared with the data of isocratic HPLC procedure on a Bondapack reversed-phase ODS or C18 Waters column (chemically bonded to Spherisorb(particles (5 μm), 4.6 mm × 25 cm,) under conditions of isocratic elution at a flow rate of 1.0 ml/min. The mobile phase composition was acetonitrile – buffer (420 : 580, v/v), pH 7.5. The buffer composition was dibutylamine, 85% phosphoric acid, and doubly distilled water (0.18 : 0.06 : 99.76, v/v), which was adjusted to pH 7.5 with 0.8 N sodium hydroxide solution. Both the proposed UVS procedure and HPLC with UV detection were carried out at 282 nm. The linear range of detection for sumatriptan succinate by means of both UVS and HPLC methods was between 21 and 39 μg/ml of sumatriptan. The UVS method is shown to be linear, reproducible, specific, sensitive and robust.
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