Development of transport systems of quinolone class antibiotics through outer membrane vesicles (OMVs) in Gram-negative bacteria

Carlos Fernando Macedo Da Silva,Marcelo Lancellotti

doi:10.20396/revpibic262018276

Abstract

Multi-resistance to antibiotics in Gram-negative bacteria has been reported in several studies, which make more effective methods of controlling and eliminating these bacteria necessary. To overcome multiresistant profiles, we used OMVs (Outer Membrane Vesicles) as carriers of levofloxacin to encapsulate and transport the drug from the extracellular medium into the cell, overcoming resistance barriers and inhibiting cell reproduction machinery. Prepackaged formulations in this manner were quite effective and, in some cases, totally inhibited bacterial growth by making the drug efficient again.

Highlights

It has been observed all over the world the emergence of bacteria with a multiresistant profile to fluoroquinolones, bactericidal agents that act by inhibiting the activity of DNA Girase and Topoisomerase IV, enzymes fundamental for the replication of bacteria
The outer membrane vesicles used in this work were obtained from Neisseria meningitidis
To test the effect of formulations on inhibition of bacterial growth, we used eight strains commonly used in antibiogram and Minimum Inhibitory Concentration (MIC) tests

Summary

Introduction

It has been observed all over the world the emergence of bacteria with a multiresistant profile to fluoroquinolones, bactericidal agents that act by inhibiting the activity of DNA Girase and Topoisomerase IV, enzymes fundamental for the replication of bacteria. Among the mechanisms of resistance are changes in the level of permeation of antibiotics in cells (KUHNKE & LODE, 2004). Resistance to the drug is not yet very great, it can become a major problem if its use is reckless: the consequence of excessive use of quinolones is the appearance of bacterial multiresistance (Heffelinger, 2000).

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