Abstract
The citrus tatter leaf virus (CTLV) is one of the most destructive citrus viral diseases worldwide. In this study, reverse transcription-recombinase polymerase amplification combined with a lateral flow dipstick (RT-RPA-LFD) assay for rapid visual detection of CTLV was established. The assay was performed at 35 ℃ in 27 min without specialised equipment. The RT-RPA-LFD assay showed high specificity to CTLV, and the sensitivity to CTLV was the same as that of quantitative RT-PCR at 3 × 103 copies/μL CTLV RNA transcripts. A total of 45 field tangor samples were tested using RT-RPA-LFD, RT-PCR, and RT-qPCR, and the results were consistent. The results demonstrated that the RT-RPA-LFD assay is a promising tool for rapid on-site CTLV detection.
Published Version
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