DEVELOPMENT OF THE TECHNOLOGY OF DEEP CULTIVATION OF A TRANSFORMED B. SUBTILIS STRAIN

Abstract

Deep cultivation of recombinant cultures in bioreactors makes it possible to accumulate a large number of specific antigens used in the production of immunobiological preparations and diagnostic test systems in a short period of time, taking into account stimulating and limiting growth factors. The main purpose of the research was to determine the influence of the factors of seed concentration, pH level and the level of dissolved oxygen on deep cultivation, with the control of accumulation kinetics by turbidimetry. During the research, it was found that the most optimal inoculation concentration is 1 million cells/cm3 with a maximum final concentration of B. subtilis 4x106 cells/cm3, the most optimal pH level is a dynamic mode from 8.0 to 6.0 with a maximum final concentration of 5x106 cells/cm3, the most optimal level of dissolved oxygen is stationary mode with a minimum oxygen level of 25%, with a maximum final concentration of 4x106 cells/cm3.Bacteriological, technological and biochemical methods were used in the work to develop the modes of deep cultivation of the transformed B. subtilis strain with control of pH parameters and the level of dissolved oxygen.The scientific significance of the research is based on the development of deep cultivation, with an assessment of external growth factors and their influence on the kinetics of accumulation of the transformed B. subtilis strain, which will provide effective conditions for the growth and production of microorganisms. The practical significance of the research is based on the possibility of optimizing the biotechnological stages of the cultivation of the transformed B. subtilis strain, which will positively affect the efficiency of submerged cultivation as the main method for the accumulation of specific antigens.