Development of the suprachiasmatic nucleus in rats during ontogenesis: Tyrosine hydroxylase immunopositive cell bodies and fibers

Abstract

This study has evaluated differentiation of tyrosine hydroxylase-immunopositive neurons in the suprachiasmatic nucleus as well as the innervation of this nucleus by tyrosine hydroxylase-immunopositive axons in rats during ontogenesis. Tyrosine hydroxylase-containing structures were detected with electron-microscopic pre-embedding immunocytochemistry at the 22nd fetal day as well as at the second, ninth and 21st postnatal days. Rare uni- and bipolar small tyrosine-hydroxylase-immunopositive neurons were observed in the suprachiasmatic nucleus both in fetuses and postnatal rats. These neurons underwent differentiation over the perinatal period that was mainly manifested in the increase of their size as well as in the development of the Golgi complex, granular endoplasmic reticulum and the onset of the dense core vesicle production. Concomitantly, tyrosine hydroxylase-immunopositive neurons, cell bodies and dendrites, became innervated by immunonegative axons first making presynapses, and, then, symmetric (Gray-type II) and asymmetric (Gray-type I) synapses. In addition to cell bodies and dendrites, tyrosine hydroxylase-immunopositive axons were regularly observed in ventral, ventrolateral and ventromedial regions of the suprachiasmatic nucleus in fetuses and postnatal rats. Tyrosine hydroxylase-immunopositive axons were observed either in simple appositions with the immunonegative neurons or making presynapses in fetuses and symmetric and asymmetric synapses in postnatal animals. The nature of the tyrosine hydroxylase-immunopositive axons and the functional significance in the suprachiasmatic nucleus in ontogenesis are discussed.