Abstract
The α-amylase gene from Bacillus amyloliquefaciens MDC1974 was cloned into the pBE-S shuttle vector and expressed extracellularly using Bacillus subtilis RIK1285. Following the optimization of fermentation conditions, a pilot-scale production of 100 liters was conducted, achieving a maximum volumetric activity of 1969 U/ml, which is 1.4 times higher than the maximum activity obtained in flask fermentation. Additionally, enzyme concentration, drying, and preservation conditions were studied and optimized.
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