DEVELOPMENT OF SURFACE OF PROTEIN CHIP FOR CHLAMYDIA PNEUMONIAE

Abstract

PURPOSE: Chlamydia pneumoniae is an important pathogen which is etiologic agent of acute and chronic infection. The diagnosis of chlamydial infection is based on serology. Current gold standard of diagnosis is MIF(microimmunoflurescence), but it is subjective and time-consuming. Recently, protein microarray using SPR(surface plasmon resonance) sensor is suggested to be a method for detection of infection. For development of protein chip for diagnosis of chlamydial infection, we investigated the morphology of surface with AFM(atomic force microscopy) on gold chip and detected interaction between antibody for Chlamydia pneumoniae and EB(elementary body) immobilized on surface by wavelength shift using SPR sensor.