Abstract
Modern biosensor technologies can provide rapid quantification of bacterial pathogens. Surface plasmon resonance (SPR) sensors are an optical platform capable of highly sensitive and specific measuring of biomolecular interactions in real-time. This label-free technology can quantify the kinetics, affinity and concentration of surface interactions. SPR sensors have been used to detect bacterial pathogens in clinical and food-related samples. This chapter discusses the fundamental theory behind SPR sensors and state-of-the-art SPR instrumentation, surface chemistries, molecular recognition elements and detection strategies, as well as specific challenges associated with bacterial detection using SPR sensors. SPR-based detections of bacterial cells, genetic markers and antibody biomarkers are reviewed and discussed.
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