Abstract
<p>Profile of molecular marker can be used for variety identification, genetic purity monitoring of germplasm and additional<br />requirement in proposing intellectual property protection. DNA fingerprinting of soybean had been applied at the ICABIOGRADIAARD<br />since 2004 using simple sequence repeat (SSR) markers which were run automatically by CEQ 8000 Genetic Analyzer<br />platform based on capillary electrophoresis system. This method had produced unique DNA fingerprints of the varieties tested,<br />but the marker set to efficiently identify the varieties had not yet been developed. This study aimed to develop a set of SSR<br />markers as a tool to identify the Indonesian soybean varieties. Fourty two soybean varieties were analyzed using 14 random SSR<br />markersA total of 168 alleles that were obtained from the polymorphism analysis. The average of polymorphic information<br />content (PIC) value observed was 0.7337 per SSR locus. Based on marker reproducibility rate, PIC value, number of rare alleles,<br />frequency of dominant alleles, and percentage of SSR fragment detected by genetic analyzer, we identified five SSR markers i.e.<br />Satt414, Satt147, Satt308, Satt009, and Satt516 as a SSR marker set to be used for soybean variety identification purposes. This<br />marker set was used to develop the identity (ID) of the 42 Indonesian soybean varieties.</p>
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