Abstract
Auricularia auricula-judae , an important edible and medicinal mushroom was first cultivated in China more than one thousand years ago. In this study, we developed a simple and rapid polymerase chain reaction (PCR)-based diagnostic tool for identifying A. auricula-judae from other morphologically similar Auricularia species. Based on the sequence analysis of intergenic spacer 1 (IGS1) in genus Auricularia , two species-specific primer combinations were designed from IGS1 sequence of A. auricula-judae , both of them can generate DNA fragments of different size unique to A. auricula-judae . The validity of this two primer combinations were confirmed by correctly amplifying species-specific fragments from genomic DNA of another 32 A. auricula-judae strains. To our knowledge, this was the first study on development of species-specific primers via IGS1 sequence in jelly fungi and also the first application of IGS1 sequence in genus Auricularia .
Published Version
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