Abstract
Different strains of rhizobia (isolated fromLotus corniculatus andVigna unguiculata) andRhizobium meliloti adapt to sevin (50 μg/ml). The number of transfers (20–31) and days of incubation (80–130) during which different strains of rhizobia develop resistance varied. The results of reversion of resistance to sevin, experiments showed that the resistance developed was stable. Rate of growth was faster in resistant strains but their final cell numbers were less than those of sensitive strains. Dehydrogenase activity increased with the development of resistance to sevin, except in strain D-467. With the development of resistance to sevin, total lipids and phospholipids decreased, glycolipids increased and neutral lipids varied. Presence of glycerol, sodium oleate and sodium acetate (known to stimulate lipid production) and flavin mononucleotide and wheat germ lipases (known to decrease lipid production) in the culture medium did not change the growth pattern and lipids of the sevin resistant and sensitive strains of rhizobia.
Published Version
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