Abstract
Objective: The main objective was to focus on qualitative and quantitative analysis of isolated okra mucilage by High-performance Thin Layer Chromatography to set up the quality control parameters for the isolated mucilage.
 Methods: High-performance thin-layer chromatography (HPTLC) were applied for the identification of components present in methanolic and ethanolic fruit extract of okra at 254 nm and 356 nm. Quantitative analysis of amino acid ascorbic acid and total polyphenol content was determined.
 Results: The results showed that the yield percentage for methanolic and ethanolic fruit extract of the okra fruit mucilage was found to be 13.5 and12.5% respectively. HPTLC determination of methanolic and ethanolic okra fruit extract showed the presence of 8 components with Rf values in the range of 0.14 to 0.62 and 0.14 to 0.54 respectively when detected at wavelengths 254 nm and at 356 nm. The total amino acid in okra fruit methanolic extract was found to be 11.45%w/w. The ascorbic acid content in methanolic okra fruit extract and ethanolic okra fruit extract was found to be 0.24 %w/w and 0.18% w/w respectively. The total phenolic contents (tannic acid equivalents, mg/g) in the methanolic and ethanolic okra fruit extracts were calculated to be 4.6 % w/wand 5.3% w/w respectively.
 Conclusion: The data revealed dual benefits like it can act as a potential novel functional ingredient with health-promoting application due to the presence of ascorbic acid and total phenolics contents and at the same time the data provided guidelines for quality control parameters for the isolated okra fruit
Highlights
Nature has blessed us with a very rich and plethora of botanical wealth and a huge number of valuable medicinal plants which play a pivotal role in modern medicine as well as in traditional medicine for centuries to treat various diseases worldwide since time immemorial [1]
The present study provides us with adequate information about the identification, standardization, and quality control of okra fruit mucilage and about the the therapeutic efficacy of the okra mucilage
This analytical method can be used for its various analysis in plant materials and in routine quality control laboratories and research zone for the raw materials as well as formulations containing this compound
Summary
Nature has blessed us with a very rich and plethora of botanical wealth and a huge number of valuable medicinal plants which play a pivotal role in modern medicine as well as in traditional medicine for centuries to treat various diseases worldwide since time immemorial [1]. Green pharmaceuticals are demanding popularity and importance and acceptance is increasing progressively because immense opportunities for new drug discoveries are provided by keen interest in plant-derived drugs due to the fact that “green medicines’’ have a valuable approach for benefits of humankind in order to search for economical ingredients and enabling companies to enhance their existing product the cost of drug development plays pivotal role in order to cope with worldwide challenges and competition. Herbal excipients are being explored and employed in dosage formulations and drug delivery research [4]. Mucilages can be classified as herbal excipients which are safe and eco-friendly, find applications in pharmaceutical dosage formulations. Mucilages find wide range of applications in biphasic liquid dosage forms, such as thickeners in oral liquids binding, disintegrating agent, suspending and emulsifying agent, stabilizing and gelling agents, film-forming agents in transdermal and periodontal films and as gel base for tropical application [5]
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