Abstract
Ayurveda is the oldest surviving complete medical system in the world. Avalehas are important group of formula- tions used by ayurvedic physicians to treat various types of diseases. Drakshavaleha is official in ayurvedic formulary of India and it consists of six reputed herbs (Piper longum, Zingiber officinale, Ribes nigrum, Glycyrrhiza glabra, Emblica officinalis and Bambusa arundinacea), of which Ribes nigrum is the prime ingredient. The formulation is dispensed for anaemia and jaundice. It is observed that the consistency and taste of the formulation varies from manufacturer to manufacturer. Three in- house batches of Drakshavaleha was prepared. These batches were evaluated for physicochemical parameters such as extractive value, ash value, loss on drying, pH, total solid content, amount of reducing sugar, fat content etc. In present communication, a TLC- method was developed for the standardization of Drakshavaleha by quantitative estimation of major antioxidant compounds, piperine, as marker. The developed method was validated with respect to linearity, precision, accuracy, and robustness. Stability study was performed according to the ICH guidelines. The results of three in- house batches were found in close proximation with each other. The method used for determination of quality control of Drakshavaleha was found to be precise and reproducible and can be used for other marketed formulations.
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