Abstract
Aim. To develop quality control methods of biologically active substances of liquid extracts from Aesculus hippocastanum seeds and Delphinium elatum roots, which are the main components of the new dosage form in the form of the gel with the venotonic action.Materials and methods. Identification of the main biologically active substances of plant extracts was carried out by chemical reactions and thin layer chromatography in the solvent system of chloroform – glacial acetic acid – methanol – water (15 : 8 : 3 : 2) using standard samples of escin and allantoin as reference samples. Detection of chromatograms was performed by treating with phosphatotungstic acid solution. The quantitative determination was carried out by absorption spectrophotometry in the visible range. Calculation of the quantitative content was performed by the standard method.Results and discussion. By chemical reactions biologically active substances of polyphenolic structure were found in the gel studied. The presence of escin and allantoin in the gel composition was confirmed by the method of thin layer chromatography compared to standard samples. The quantitative determination of the amount of triterpene saponins calculated with reference to escin was performed by spectrophotometry in the visible range, and it was from 32 mg to 42 mg in the gel.Conclusions. The results obtained when performing chemical reactions, the method of thin-layer chromatography and the quantitative determination by absorption spectrophotometry allow recommending the dosage form studied for standardization by the amount of triterpene saponins calculated with reference to escin.
Highlights
To date, drugs for external use are becoming more widespread
Among them a particular place is occupied by soft dosage forms such as gels, ointments, creams and others
Quality control methods were previously developed on model mixtures for which the extracts studied and gel excipients were used
Summary
Drugs for external use are becoming more widespread. Among them a particular place is occupied by soft dosage forms such as gels, ointments, creams and others. A special attention among soft dosage forms should be given to gels that have high rheological properties, the penetrating ability and ease of use. It should be noted that the composition of gels includes both chemical substances and medicinal herbal products as active pharmaceutical ingredients. At the Department of Industrial Drugs Technology of the National University of Pharmacy a new dosage form in the form of a gel with the marked anti-inflammatory, membrane-stabilizing and antithrombotic activity has been developed [1]. The pharmacological effect of the dosage form proposed is achieved due to the presence of two plant extracts – a liquid extract from Aesculus hippocastanum seeds and a liquid extract from Delphinium elatum roots
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