Development of Plant-Based Larvicide and Herbal Mosquito Repellent Fast Card with Reference to Identification of the Functional Bioactive Compounds Effective Against Culex Mosquito.

Amit Chattopadhyay,Subarna Ghosh,Pranab Kr Banerjee,Piyali Mukherjee,Vikky Shaw

doi:10.1007/s12010-022-03798-8

Amit Chattopadhyay, Subarna Ghosh + Show 3 more

Open Access

https://doi.org/10.1007/s12010-022-03798-8

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Abstract

Warm and humid climate creates ideal conditions for mosquito breeding. The ability of these vectors to spread a number of diseases to humans causes millions of deaths every year. Indiscriminate use of synthetic insecticides leads to the development of resistance in vector mosquitoes and along with this, these pesticides cause biological magnification of toxic components and affects adversely the non-target organisms including human being. Commercially available, chemically manufactured mosquito repellent fast cards are convenient to use and quite effective but burning of such card generates a lot of smoke and might be hazardous to human health in the long run. Thus, alternative approaches are to be adopted to control the population load of vector mosquito. Like that, the present study also reveals the larvicidal effect of Duranta leaf extract against Culex mosquito. In the present study, mosquito repellent fast card has been developed by Duranta-algal mixture which has shown better result than commercially available fast card on the basis of mosquito mortality as well as the amount of gases emitted. Again, the ethanolic crude extract of Duranta leaves leads to 100% mortality of all instars (Culex pipiens) larvae at both 1000ppm and 500ppm concentration. Therefore, the active component of Duranta has also been investigated. In Duranta, highest area percentage and peak have been shown by propionic acid in the retention time 18.086 by GC-MS. So, it can be confirmed that the major active ingredient is propionic acid in Duranta which is responsible for the mosquitocidal properties. Occurrence of propionic acid in Duranta has also been confirmed by the HPLC analysis.

Highlights

A number of diseases like Dengue, Malaria, Filariasis, Japanese encephalitis, West Nile fever are transmitted among humans and animals mainly by 4 genera of mosquitoes viz. Culex sp
The present study reveals the larvicidal effect of Duranta leaf extract against Culex mosquito
Duranta repens are locally available provides means to protect the human health from vector mosquitoes

Summary

Introduction

A number of diseases like Dengue, Malaria, Filariasis, Japanese encephalitis, West Nile fever are transmitted among humans and animals mainly by 4 genera of mosquitoes viz. Culex sp. Public concern has increased significantly regarding their negative impacts such as potential health hazards, water contamination, environmental pollution, toxicity to non target organism including man and the development of resistance in mosquito [4, 5]. The active toxic ingredients of plant extracts are secondary metabolites which are developed to defend the plant themselves from primary consumers or herbivores These toxic phytochemicals have a number of physiological and biochemical targets in the insect body. These targets are proteins, nucleic acids and other cellular elements This in turn, hampers normal physiological activities of insect body in different ways, the principal of which is the development of abnormality in the nervous system [13]. In the present study the active ingredient of Duranta leaf extract has been identified for its further use as Culex controlling agent

Materials And Methods

Findings

Discussion