Development of phytoremediator screening strategy and exploration of Pennisetum aided chromium phytoremediation mechanisms in soil

Abstract

Screening of chromium (Cr) phytoremediators (i.e., hyperaccumulator plants and accumulation plants) is essential for the phytoremediation of Cr-contaminated soils but less tackled previously. In this study, we proposed a stepwise strategy for screening Cr phytoremediators and explored tolerance mechanism of the screened species. To achieve effective screening of Cr phytoremediators, seed germination, hydroponic, and pot experiment were performed sequentially, and an improved indicator system was established accordingly. Pennisetum was selected from nine plants, with its high growth rate and Cr remediation efficiency successfully demonstrated in the field. Antioxidant enzymes (i.e., superoxide dismutase (SOD) and catalase (CAT)) and photosynthesis under Cr stress were monitored for tracking the tolerance mechanism. Results showed that the enhanced SOD and CAT contributed to the strong tolerance of Pennisetum to Cr. The SOD and CAT were positively correlated with net photosynthetic rate (Pn), resulting in a phenomenon that Cr had no significant effect on Pn of Pennisetum even at 400 mg kg−1. The research findings helped obtain powerful Cr phytoremediators, deepen our understanding of the tolerance mechanisms associated with phytoremediation, and eventually facilitate effective Cr removal in soil.