Abstract
This study satisfies the primary need for a simple monitoring scheme for antibiotic residue content in freshwater systems to regulate and prevent immunization of aquaculture species to antibiotics. A series of antibiotic solutions with decreasing concentrations (from 5ppm to 1ppm) were prepared, with each solution containing fixed amounts of the reagents (p-Chloranilic acid in acetonitrile solution, and pure acetonitrile) and a buffer solution to distinctly simulate the environmental conditions of actual freshwater systems. Using the UV-Vis Spectrophotometer with the optimum wavelength of 519nm, the stability, repeatability, and sensitivity tests were performed, where relative standard deviation (RSD) values of less than 10% were obtained. The limit of detection was also calculated to be 0.54ppm, using the RSD result from the sensitivity test. Actual freshwater sample was gathered and subjected to a repeatability test using the same working wavelength. Results showed that the trend in the absorbance reading for the real water sample differed with the laboratory standard samples. This shift may have been caused by other interfering chemicals used in aquaculture that were present in the sample. Modified detection method of Oxytetracycline (OTC) in freshwater was made to provide solution to the main objective.
Highlights
Dubbed as the “Center of Marine Biodiversity” in the world, Philippines is known for its sanctified waters, providing home to millions of aquatic species, some of which are only found here in the country
The general objective of this study is to provide the aquaculture industry a method for antibiotic concentration determination
The stability, repeatability, sensitivity, and limits of detection of the method were obtained, and relative standard deviation (RSD) values of less than 10% for each of the parameters are achieved
Summary
Dubbed as the “Center of Marine Biodiversity” in the world, Philippines is known for its sanctified waters, providing home to millions of aquatic species, some of which are only found here in the country. This interesting fact paved way for the growth and development of the fisheries industry in the country. As a Antibiotics are widely used in human and veterinary medicines, in agriculture, and in aquaculture as growth promoters [4]. This has become a necessity due to its various impacts in ecosystem functioning, as well as in public health. Contamination of veterinary drugs in aquatic environment has become an increasingly important and alarming issue since these residues remotely increase the population of drugresistant bacteria and further promote antibioticresistance infection to aquaculture species [1]
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