Abstract
The current investigation is focused on formulation, optimisation and characterisation of polymeric based nanomaterial. Nitazoxanide (NTZ) loaded polymeric nanoparticles were prepared by homogenisation technique using Eudragit RL100 as a polymer matrix and Poly vinyl alcohol (PVA) as a cross linking agent. NTZ was used as a model drug and investigated for preformulation parameters along with excipients, identification of concentration for optimization, selection of independent (X) and dependent (Y) variables and characterisation of optimised formulation. Polymeric nanoparticles were obtained after optimization using 33 factorial design by Box Behnken Design expert (BBD). The role and influence of key process variables i.e. concentration of polymer, concentration of cross linking agent and speed of rotation of homogeniser at their respective three different levels for the optimisation of formulation were also investigated. The synthesised optimised polymeric nanoparticles were further characterised by dynamic light scattering (DLS) for its particle size (137.11nm), PDI (0.180) and zeta potential (33.4 mV) while X-ray diffraction (XRD) was used to justify the amorphous and crystalline nature of drug and excipients. Transmission electron microscopy (TEM) further revealed surface geometry of these nanoparticles being spherical in shape, drug entrapment efficiency (%DEE) was found to be 81.89% and in vitro release studies showed sustained drug release effect. The antimicrobial activity against Pseudomonas aeruginosa, Streptococcus mutans and Escherichia coli was also determined.
Highlights
As per the estimated data given by WHO for parasitic infections, there are 48.4 million cases and 59,724 deaths annually announced in 8.78 million (Disability Adjusted Life Years) DALYs
In the above research work we synthesised the polymeric nanoparticles by homogenisation technique
Multivariate investigational design projected a quadratic model as the efficient relationship between the dependent variables (i.e particle size, polydispersive index (PDI), zeta potential) and stated by response surface methodology
Summary
As per the estimated data given by WHO for parasitic infections, there are 48.4 million cases and 59,724 deaths annually announced in 8.78 million (Disability Adjusted Life Years) DALYs. The disease load from parasites is extremely central and results in substantial morbidity and mortality between susceptible populations, [1] we have to focus on the novel formulation development specially nanomaterials using parasitic agent as a model drug. These type of alteration in formulation of the material in nanotechnology often yield a product with improved prospective as well as inspire the researchers through the diverse disciplines to further exploration of nanomaterials in different pharmaceutical field. Polymeric nanoparticles utilized Eudragit RL100 as a polymer and polyvinyl alcohol (PVA) as cross linking agent using NTZ as a model drug. The pharmacological activity of NTZ is supposed due to interference with pyruvate-ferrodoxin oxidoreductase (PFOR), enzyme dependent electron transfer reaction which is essential to anaerobiotic energy breakdown
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