Abstract
Moniliophthora perniciosa is a fungus that causes witches' broom disease (WBD) in the cacao tree (Theobroma cacao). The M. perniciosa genome contains different transposable elements; this promp- ted an evaluation of the use of its retrotransposons as molecular markers for population studies. The inter- retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymor- phism (REMAP) techniques were used to study the variability of 70M. perniciosa isolates from different geographic origins and biotypes. A total of 43 loci was amplified. Cluster analysis of different geo- graphical regions of C biotype revealed two large groups in the state of Bahia, Brazil. Techniques using retrotransposon-based molecular markers showed advantages over previously used molecular techniques for the study of genetic variability in M. perniciosa.
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