Abstract
Karyotype analysis of 3 released varieties in Vigna radiata L. viz. Barimung-2, Barimung-3, Barimung-5 has been carried out after staining with orcein, CMA and DAPI. These varieties were found to possess 2n=22 chromosomes. Four terminal CMA-positive bands were found in Barimung-2, 3 such bands in Barimung-3 and 4 in Barimung-5. The terminal CMA positive bands showed deep DAPI-negative reversible bands in the 3 varieties of V. radiata. One chromosome of Barimung-5 was fluoresced entirely with CMA and DAPI. DAPI-positive and DAPI-negative band occurred in another chromosome of Barimung-5. A pair of interstitial CMA-negative band was observed in chromosome pair V of only Barimung-2. These portions were stained brightly with DAPI. These kinds of reversible banded chromosomes could be easily identified. Polymorphism regarding the number and location of DAPI-positive bands were observed in these varieties. That may due to either minute deletion or high condensation of heterochromatic region at the respective loci. Few chromosomes of each variety showed characteristic CMA and DAPI bands. Therefore, with the help of CMA and DAPI it was possible to develop marker chromosomes for authentic identification of three varieties in V. radiata.
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