Development of Ionic Liquid Modified Disposable Graphite Electrodes for Label-Free Electrochemical Detection of DNA Hybridization Related to Microcystis spp.

Ceren Sengiz,Gulsah Congur,Arzum Erdem

doi:10.3390/s150922737

Ceren Sengiz, Gulsah Congur + Show 1 more

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https://doi.org/10.3390/s150922737

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Journal: Sensors (Basel, Switzerland)	Publication Date: Sep 9, 2015
Citations: 55	License type: CC BY 4.0

Affiliation: Ege University

Abstract

In this present study, ionic liquid (1-butyl-3-methylimidazolium hexafluorophosphate (IL)) modified pencil graphite electrode (IL-PGEs) was developed for electrochemical monitoring of DNA hybridization related to Microcystis spp. (MYC). The characterization of IL-PGEs was performed using microscopic and electrochemical techniques. DNA hybridization related to MYC was then explored at the surface of IL-PGEs using differential pulse voltammetry (DPV) technique. After the experimental parameters were optimized, the sequence-selective DNA hybridization related to MYC was performed in the case of hybridization between MYC probe and its complementary DNA target, noncomplementary (NC) or mismatched DNA sequence (MM), or and in the presence of mixture of DNA target: NC (1:1) and DNA target: MM (1:1).

Highlights

Nucleic acid analysis using biosensing strategies has been an attracting topic in many fields including gene analysis, clinical disease diagnosis, biological, environmental, pharmaceutical and forensic applications [1,2,3,4,5,6,7,8]
Ionic liquids (ILs) modified disposable pencil graphite electrodes (PGEs) were developed and label-free detection of sequence selective DNA hybridization related to Microcystis spp. was performed by using IL-PGEs
The microscopic and electrochemical characterizations of IL-PGEs in contrast to unmodified PGEs were successfully done via SEM, electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques, respectively

Summary

Introduction

Nucleic acid analysis using biosensing strategies has been an attracting topic in many fields including gene analysis, clinical disease diagnosis, biological, environmental, pharmaceutical and forensic applications [1,2,3,4,5,6,7,8]. Ionic liquids (ILs) are known as low-melting organic salts and mostly liquid at room temperature Due to their unique properties such as low measurable vapor pressure, high thermal stability and conductivity, having good solvating properties, non-volatility, low toxicity and biocompatibility, they have been used in different fields including development of electrochemical biosensors [13,21,22,23,24,25,26,27,31,32,33]. Ren et al [32]

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