Development of In-Vitro Xylanase Digestion (IVXD) Strategy Using Agriculture Wastes for the Production of Exopolysaccharide Through Solid-State Fermentation Using Lactobacillus plantarum MTCC 2621

Abstract

In this research work, the study of exopolysaccharides, structure and amount of the exopolysaccharides (EPS) and these are produced by Lactic acid bacteria (Lactobacillus plantarum and Bacillus licheniformis), plants, and fungus. Lactic acid bacteria are gram-positive and non-sporulating that ferment various carbohydrates like as lactate and acetate and various advantages of this, like as EPSs are economically important because exopolysaccharides have many applications in the food industries, pharmaceutical industries, and other industries and eps production, enzyme production, organic acid production by the solid-state fermentation. Solid-state fermentation (SSF) defines the growth of microorganism without free-flowing aqueous phase. In the solid-state fermentation, wheat bran and pretreatment substrate were used like as rice straw (alkali pretreatment) and acid (pretreatment). Almost known microbial enzymes can be produced by the solid-state fermentation techniques. Enzymes of industrial importance, like cellulose, protease, pectinases, xylanases, amylase, glucoamylases, etc. used the solid-state fermentation. In this research work, the production of the exopolysaccharides (EPS) and to check the xylanase activity from the different-different methods these are phenol-sulphuric acid, Lowry method and di-nitro salicylic (DNS) acid method.