Bioconversion of Agricultural Waste to Butyric Acid Through Solid State Fermentation by Clostridium tyrobutyricum

Abstract

The present study was aimed to develop a successful bioprocess through solid state fermentation (SSF) technique for the butyric acid production using agriculture wastes like wheat bran, rice polishings and molasses anaerobically by Clostridium tyrobutyricum DSM 2637. Different physiochemical parameters like substrate: water ratio, inoculum volume, different ionic salts concentrations, an additive effect of molasses and corn steep liquor were optimized to boost the butyric acid production through SSF of the growth medium anaerobically by Clostridium tyrobutyricum DSM 2637 at 37 °C, pH 6.0. The fermentation of the growth medium was terminated after 72 h of incubation. Quantification of butyric acid was done by Deniges method. The growth medium containing rice polishings, molasses, and wheat bran when subjected to determine substrate: water ratio through SSF at 37 °C, pH 6.0 anaerobically by Clostridium tyrobutyricum DSM 2637 yielded 5.63, 5.5, 4.26 mg/100 g of butyric acid respectively. The present investigation demonstrated that rice polishings supplemented with corn steep liquor is a good agricultural waste for butyric acid production. The SSF technique can efficiently and successfully be utilized for better yield of butyric acid on the pilot scale.