Abstract
In this study, polyaniline-multiwalled carbon nanotube film (PANi–MWCNT) has been polymerized on interdigitated platinum electrode arrays (IDA), fabricated by MEMS technology for the detection of human papillomavirus (HPV) infection, using immobilized peptide aptamers as affinity capture reagent. Label-free, electrochemical detection of the specific immune reaction between antigen peptide aptamer HPV-16-L1 (with a molecular weight of 1825Da), the most common genotype in cytological normal women worldwide, and its specific antibody of HPV-16 (which is much bigger with molecular weight of ca. 150kDa) on multifunctional PANi–MWCNT based arrays was reported. The most significant advantage of this technique consists of reagentless and multiple detection of antigen–antibody complex formation on well conducting IDA interface of PANi–MWCNT, without intermediate steps or any labeling reagents, as normally required in the previous works.
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