Abstract
The aim of this work was the development of pH-sensor-NP allowing the quantification of the amount of NP phagocytosed by macrophages. Two types of fluorescent NP with variable and well-characterized sizes and chemicals coatings have been synthesized:NP with a FITC core (FITC-NP): green fluorescence (control).FITC-NP functionalized with a pH sensitive probe (pH-sensor-NP): green fluorescence of the FITC and red fluorescence of the pH probe in acidic conditions.Our pH-sensor-NP model was first validated in acellular conditions. They were then incubated with a macrophage cell line allowing distinction and quantification of internalized NP with no major effects on biological toxicity.
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