Abstract
Skin wound repair requires the development of different kinds of biomaterials that must be capable of restoring the damaged tissue. Type I collagen and chitosan have been widely used to develop scaffolds for skin engineering because of their cell-related signaling properties such as proliferation, migration, and survival. Collagen is the major component of the skin extracellular matrix (ECM), while chitosan mimics the structure of the native polysaccharides and glycosaminoglycans in the ECM. Chitosan and its derivatives are also widely used as drug delivery vehicles since they are biodegradable and noncytotoxic. Regulation of the inflammatory response is crucial for wound healing and tissue regeneration processes; and, consequently, the development of biomaterials such as hydrogels with anti-inflammatory properties is very important and permissive for the growth of cells. In the last years, it has been shown that mesenchymal stem cells have clinical importance in the treatment of different pathologies, for example, skin injuries. In this paper, we describe the anti-inflammatory activity of collagen type 1/chitosan/dexamethasone hydrogel, which is permissive for the culture of human adipose-derived mesenchymal stem cells (hADMSC). Our results show that hADMSC cultured in the hydrogel are viable, proliferate, and secrete the anti-inflammatory cytokine interleukin-10 (IL-10) but not the inflammatory cytokine Tumor Necrosis Factor-alpha (TNF-α).
Highlights
The mechanisms involved in the tissue regeneration process can be used to design successful treatments for several pathologies and lesions
We describe the anti-inflammatory activity of collagen type 1/chitosan/dexamethasone hydrogel, which is permissive for the culture of human adipose-derived mesenchymal stem cells
Our results show that human adipose-derived mesenchymal stem cells (hADMSC) cultured in the hydrogel are viable, proliferate, and secrete the anti-inflammatory cytokine interleukin-10 (IL-10) but not the inflammatory cytokine Tumor Necrosis Factor-alpha (TNF-α)
Summary
The mechanisms involved in the tissue regeneration process can be used to design successful treatments for several pathologies and lesions. Interleukin-10 (IL-10) is an anti-inflammatory cytokine that has been related to the regeneration capacity; for example, skin lesions in IL-10 knockout mice embryos result in the formation of scar tissue in the wounded area [1]. Hubner and collaborators showed that the application of Dex immediately after skin injury resulted in a decrease of the inflammatory cytokines and the keratinocyte growth factor, which causes a deleterious response of the glucocorticoid on wound repair [20]. These reports suggest that the concentration, environment, and postinjury time at which Dex is applied are crucial in wound healing, influencing whether scar reparation or regeneration is observed. These model hydrogels might have potential applications in wound healing
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
