Adhesion, proliferation and differentiation of human adipose mesenchymal stem cells on PLGA

Abstract

Objective To study the ability of adhesion, proliferation and differentiation of human adipose mesenchymal stern cells,which were cultured in PLGA,and thus to provide a basis for their in vivo implantation. Methods PLA was mixed with PGA in a ratio of 1 ： 1, and PLGA was obtained by insert moldling with organic solvents. In vitro, human adipose mesenchymal stem cells were adhered to PLGA. The attachment and the proliferation of human adipose mesenchymal stem cells in PLGA were analysed by scanning electron microscope, and the morphological change of the cells was investigated before and after induction. Results Human adipose mesenchymal stem cells were able to attach, proliferate and secrete extracellular matrix in porous PLGA （pore size： 100 -400 pm） with porosity of 85 % , and to differentiate into round lipoblast-like cells under the induction of adipose medium. After 14 days, the particulate of PLGA was overlaid by extracellular matrix. Conclusion Human adipose mesenchymal stem cells have the capacity to adhere to PLGA and proliferate, indicating that PLGA can be used as a carrier for human adipose mesenchymal stem cells in adipose tissue engineering. Key words: Human adipose mesenchymal stem cells; Scaffold material; Scanning electron microscope