Abstract
Centella asiatica (Apiaceae) is a tropical/subtropical medicinal plant, which contains a variety of triterpenoids, including madecassoside, asiaticoside, madecassic acid, and asiatic acid. In this study, we tested the efficiency of hairy root (HR) induction in C. asiatica from leaf and petiole explants. Leaves and petioles collected from C. asiatica plants were suspended in agro-stock for 30 min and co-cultured with Agrobacterium rhizogenes for 3 days to induce HR formation. The transformation efficiency of leaf and petiole explants was approximately 27% and 12%, respectively. A total of 36 HR lines were identified by PCR-based amplification of rol genes, and eight of these lines were selected for further analysis. Among all eight HR lines, the petiole-derived lines HP4 and HP2 displayed the highest growth index (37.8) and the highest triterpenoids concentration (46.57 mg∙g−1), respectively. Although triterpenoid concentration was >2-fold higher in leaves than in petioles of C. asiatica plants, the accumulation of triterpenoids in petiole-derived HR cultures was 1.4-fold higher than that in leaf-derived HR cultures. Additionally, in both leaf- and petiole-derived HR cultures, terpenoid production was higher in HRs than in adventitious roots. These results demonstrate that the triterpenoid content in the explant does not affect the triterpenoid content in the resultant HRs. The HR culture of C. asiatica could be scaled up to enable the mass production of triterpenoids in bioreactors for the pharmaceutical and cosmetic industries.
Highlights
Induction and Characterization of hairy root (HR) In C. asiatica, HRs were obtained from leaf and petiole explants obtained from in vitrogrown plantlets by co-cultivation with A. rhizogenes strain KCCM 11879
Multiple HRs were induced from main vein and most of them were directly induced from the explant (Figure 1)
The present study showed that hairy roots were an effective system to produce triterpenoid from C. asiatica, and the results indicated that the content in the source tissue does not affect the triterpenoid content in the HRs and Adventitious roots (ARs) of C. asiatica
Summary
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Centella asiatica belongs to the Apiaceae family (Figure S1) and is a perennial, prostrate, herbaceous creeper plant. The stems are slender, with long stolons to connect plants to each other [1]. C. asiatica is a medicinal plant native to the tropical and subtropical countries of
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