Development of Growth Medium for Centella Asiatica Cell Culture Via Response Surface Methodology

Rozita Omar,M.A Hasan,M.A Abdullah,M Marziah

doi:10.3844/ajassp.2004.215.219

Abstract

The effects of sucrose, Indole-3-Acetic Acid (IAA) and 6-BenzylAminoPurine (BAP) concentrations on cell growth of Centella asiatica cell suspension culture were studied. The concentrations were designed using Central-composite experimental design and regression analysis was carried out to obtain response surface model describing cell growth for prediction of optimum conditions. Only sucrose as a single factor was positively significant for cell growth. Increasing sucrose concentration from 3.32 to 6.68% (w/v) resulted in an increase in dry cell weight from 16-27 g L-1. IAA and BAP as single factors and other possible interaction effect were insignificant. The optimum values predicted to be 6.68% (w/v) sucrose, 0.84 mg L-1 IAA and 1.17 mg L-1 BAP yielding 27.4 g L-1 dry cell weight with 81.4% regression equation fitness of the experimental data.

Highlights

Metabolism, growth and development[3]
A sucrosefed cell culture is probably similar to the whole plant in Interest in pharmaceutical products and drugs terms of its primary metabolic pathways[4] and the level derived from plants has increased tremendously in of carbon source affects both cell growth and product recent years
The effects of terpenoid synthesis, genetic or metabolic engineering of Plant Growth Regulators (PGRs) depend on diverse factors such as plant plant terpenoid metabolism presents a venue for studies species, hormone type and concentration[5]

Summary

Introduction

Metabolism, growth and development[3]. A sucrosefed cell culture is probably similar to the whole plant in Interest in pharmaceutical products and drugs terms of its primary metabolic pathways[4] and the level derived from plants has increased tremendously in of carbon source affects both cell growth and product recent years. The little effect of cytokinin is found in availability of sugars or their derivatives Cinchona succirubra cell growth and anthraquinone initiate different responses and would affect plant formation[9]. Since secondary metabolites are unique to each plant species, strategies must be devised for optimization of medium and cultural conditions for optimum cell growth and secondary metabolite production.

Results

Conclusion