Development of functionalized polymeric nanomaterial by organosiliconic reagent for boronate affinity-based dopamine recognition

Abstract

The dopamine neurotransmitter is mainly responsible for endocrine functions in our body. The increase in the level of dopamine in the human body leads to many disorders such as schizophrenia, uncontrollable tics, addiction, increased tension, desire to act excessively, and carelessness. Decreased levels of dopamine cause problems such as malnutrition, stress, insomnia, and the use of antidepressants. Recognition of dopamine is crucial for clinical experiments. Therefore, the development of recognition surfaces of dopamine is essential for isolation, purification, and determination processes. This study, it was aimed to produce a polymeric nanomaterial that can recognize dopamine. For this purpose, p(HEMA) polymer was synthesized with surfactant-free emulsion polymerization method and silanized by organosilicon reagent (3-aminopropyl) triethoxylsilane (APTES) and modified with phenylboronic acid (PBA). Characterization of the p(HEMA)-APTES-PBA nanopolymeric system for dopamine recognition was performed with Scanning Electron Microscope(SEM), Energy Dispersive Spectrometry (EDS), Fourier Transform Infrared Spectroscopy (FTIR), Zeta-size/zeta-potential analysis, surface area calculations. Dopamine affinity of the p(HEMA)-APTES-PBA was optimized in terms of pH, time, concentration, buffer concentration, and buffer type parameters. Dopamine adsorption of p(HEMA)-APTES-PBA nanopolymers was 4,8 times more than epinephrine in specificity analysis. After the 7 adsorption–desorption cycles, the desorption rate was found to be 74.8 %. The developed nanopolymeric system is a convenient method with high adsorption capacity which allows easy and rapid extraction, isolation, and purification of dopamine.